Modification of Zn-Metal Organic Framework with Nano Silver as an Antibacterial Material for Diabetic Ulcers (In vitro)

Zn-MOF synthesis was carried out using the solvothermal method with DMF as the solvent, and Zn-MOF was modified with AgNPs to produce Ag/Zn-MOF using ultrasonication. XRD analysis results indicate that Ag(10)/Zn-MOF and Ag(20)/Zn-MOF exhibit diffraction patterns similar to Zn-MOF but with some shifts and the addition of new peaks at 2? around 38° and 45°, characteristic of Ag2O and AgO. FTIR analysis confirms the successful synthesis of Zn-MOF, as evidenced by characteristic functional group vibrations observed in the ATR-FTIR spectrum, such as -COOsym (1584 cm-1) and -COOasym (1393 cm-1), indicating the bonding between carboxylate ligands and the central Zn metal, as well as Zn-O vibration (646 cm-1), indicating the presence of Zn4O metal clusters. After modification with AgNPs, an Ag-O peak is also detected (516 cm-1). The SEM-EDX analysis results indicate that Ag(20)/Zn-MOF has a cubic shape with 265-193nm dimensions. Its form is heterogeneous, irregular, and rough, with AgNP aggregates measuring around 20-50nm on the surface. TGA analysis results show that modifying AgNPs can enhance the thermal stability of Zn-MOF. Quantum chemical characteristics by the DFT method show that Ag/Zn-MOF is an excellent electron acceptor, which can increase the production of Reactive oxygen species (ROS), which can increase antibacterial properties.The antibacterial activity (in vitro) analysis data reveals that Ag(20)/Zn-MOF exhibits the highest inhibitory effect against ulcer-causing bacteria. In conclusion, this study demonstrates that Ag(20)/Zn-MOF is effective as an antibacterial material for diabetic ulcers.